ABSTRACT
Objective:To explore the role of TLR4 in the process of lower limb ischemia-reperfusion injury.Methods:Ischemia Reperfusion injury model:in this study,the model of lower limb ischemia-reperfusion was established by rubber band wound method,C57BL / 6J mice were randomly divided into model control group(IR) and ischemia-reperfusion group(IR);TLR4 experiment:The mice models of ischemia reperfusion were divided into wild type (WT) model group and TLR4 knock out (TLR4-/-) model group;The expressions of TLR4 and HMGB1,TNF-α and IL6 were detected by RT-PCR and immunohistochemistry.The apoptosis of vascular wall was detected by TUNEL.Results:The expression of TLR4,HMGB1,TNF-α,IL6 in the IR group was significantly higher than that in the control group (P＜0.01).After TLR4 knockout,the expression of inflammatory factors and apoptosis in IR group was significantly reduced (P＜0.01).Conclusion:TLR4 mediates inflammatory response and induces apoptosis in lower limb ischemia reperfusion injury in mice.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between moderate-severe asthma and development in children.</p><p><b>METHODS</b>A total of 389 children in the state of moderate-severe persistent asthma were enrolled, which were divided into moderate (226 cases) and severe group (163 cases). According to age, each group was divided into three groups: 3-year-old (85 cases and 63 cases), 5-year-old (76 cases and 52 cases) and 7 to 10 year-old (65 cases and 48 cases). Meanwhile, 298 healthy children in the same age group were enrolled as control, of which 3-year-old were 96 cases, 5-year-old were 92 cases and 7 to 10 year-old were 110 cases. Height, weight and lung function were measured respectively.</p><p><b>RESULTS</b>3-year-old of severe group, the value of height, the value of weight, the percentage of height, the percentage of weight, the SDS of height, the SDS of weight [(98.54 ± 7.75) cm, (14.87 ± 2.46) kg, 50.30% ± 16.31%, 50.27% ± 18.29%, 0.11 ± 0.66, 0.06 ± 0.49, respectively] were lower than the moderate group of the same age group ((103.58 ± 5.48) cm, (16.60 ± 2.21) kg, 65.80% ± 18.54%, 65.10% ± 18.92%, 0.46 ± 0.53, 0.45 ± 0.54, respectively) and the control group ((105.60 ± 6.29) cm, (17.82 ± 2.82) kg, 72.37% ± 11.37%, 71.92% ± 2.82%, 0.66 ± 0.62, 0.66 ± 0.52), the difference was significant (F values were 7.295, 8.034, 15.246, 10.745, 8.026, 10.864, respectively, P < 0.05).5-years of severe group, the value of height, the value of weight, the percentage of height, the percentage of weight, the SDS of height, the SDS of weight ((110.10 ± 7.36) cm, (18.76 ± 3.20) kg, 45.86% ± 18.92%, 41.69% ± 12.50%, -0.95 ± 0.42, -0.23 ± 0.34, respectively) were lower than the moderate group of the same age group ((117.76 ± 6.35) cm, (21.63 ± 2.75) kg, 61.81% ± 20.75%, 61.79% ± 18.92%, 0.36 ± 0.62, 0.38 ± 0.56) and the control group ((119.90 ± 5.78) cm, (22.80 ± 3.07) kg, 68.97% ± 18.59%, 66.27% ± 18.35%, 0.57 ± 0.65, 0.48 ± 0.63), the difference was significant (F values were 8.351, 7.864, 15.037, 13.921, 12.116, 11.725, respectively, P < 0.05).7 to 10 years-old of severe group, the value of height, the value of weight, the percentage of height, the percentage of weight, the SDS of height, the SDS of weight ((123.50 ± 9.52) cm, (23.82 ± 5.72) kg, 45.81% ± 15.51%, 42.63% ± 14.91%, -0.06 ± 0.48, -0.02 ± 0.61, respectively) were lower than the moderate group of the same age group ((129.1 ± 8.41) cm, (26.70 ± 5.72) kg, 66.84% ± 16.09%, 64.07% ± 18.58%, 0.48 ± 0.46, 0.42 ± 0.49) and the control group ((131.87 ± 7.71) cm, (28.06 ± 6.01) kg, 71.44% ± 12.70%, 69.64% ± 16.20%, 0.60 ± 0.43, 0.60 ± 0.51), the difference was significant(F values were 6.136, 6.678, 57.316, 37.893, 37.210, 34.152, respectively, P < 0.05). 3-, 5-, 7 to 10 year-old of moderate group, the value of height, the value of weight, the percentage of height, the percentage of weight, the SDS of height, the SDS of weight dropped compared to the control group of the same age, but no significant difference was found (t values were -2.008, -1.988, -1.810, -1.879, -1.713, -1.844, -1.904, -2.019, -1.605, -1.017, -1.411, -0.713, -1.881, -1.896, -1.746, -1.906, -1.523, -1.864, respectively, P > 0.05).</p><p><b>CONCLUSION</b>The height and weight of children with severe asthma were lower than those of normal children or with moderate asthma.</p>
Subject(s)
Child , Child, Preschool , Female , Humans , Male , Asthma , Body Height , Body Weight , Case-Control Studies , Child DevelopmentABSTRACT
<p><b>OBJECTIVE</b>To investigate the association of forced expiratory volume in 1 second (FEV1) and the maximum peak expiratory flow (PEF) with small airway function in asthmatic children of different ages and genders.</p><p><b>METHODS</b>This cross-sectional study was conducted among 619 asthmatic children with disease remission aged 3 to 13 years. The children were divided into 3 age groups, namely 3 to 5 years group (314 cases), 6 to 9 years group (207 cases) and 10 to 13 years group (98 cases), and their respiratory physiological parameters such as FEV1 and PEF were measured.</p><p><b>RESULTS</b>Of the airway function parameters, PEF showed the highest abnormality rate (>85%) in these asthmatic children. In male and female asthmatic children aged 6 to 9 years, abnormalities in forced expiratory flow rate 25% (MEF25) showed the highest frequency (56% and 63%, respectively). In 3-5 years and 10-13 years groups, MEF25 abnormalities were the most frequent in male children (43% and 71%, respectively), whereas abnormalities in MEF50 were the most common in female children (33% and 69%, respectively). FEV1 and PEF were positively correlated to all the parameters of small airway functions in these asthmatic children (r>0.5, P<0.01) except for MEF25 in female asthmatic children aged 3 to 5 years (r=0.19, P=0.168; r=0.086, P=0.535).</p><p><b>CONCLUSION</b>In asthmatic children, FEV1 and PEF are positively correlated to the parameters of small airway function with only the exception of MEF25 in female children aged 3 to 5 years, suggesting the value of FEV1 in the diagnosis of asthma in children.</p>
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Asthma , Diagnosis , Bronchi , Cross-Sectional Studies , Forced Expiratory Volume , Physiology , Maximal Expiratory Flow Rate , Physiology , Respiratory Function TestsABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of tracheal reconstruction with a prosthesis made of memory alloy coated with collagen sponge in mongrel dogs.</p><p><b>METHOD</b>The basic skeleton of the prosthesis was knitted with Ni-Ti memory alloy wires. The tubular mesh was sealed with polyurethane membrane and then inner and external walls of the lumen were coated with collagen sponge. Cervical trachea segmental reconstruction was performed in 8 mongrel dogs with these prostheses. The efficacy of the implanted prostheses were periodically evaluated after operation using x-ray, tracheoscopy and specimen microscope examinations.</p><p><b>RESULTS</b>One dog died of prosthesis dislocation 10 days after operation, another was killed 45 days later because of anastomotic stenosis. 6 dogs survived more than 90 days and the longest one lived for 150 days. Its implanted prosthesis was completely incorporated with the recipient tissue, where re-epithelialization occluded on anastomotic sites. The tracheal lumen was patent.</p><p><b>CONCLUSION</b>This memory alloy tracheal prosthesis has been proved useful for reconstruction of large, circumferential tracheal defects, although its long-term safety and efficiency need to be confirmed.</p>